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nanog  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc nanog
    Exosomal circ0000549 tumorigenicity, EMT, proliferation and stemness. (A) The downregulation of circ0000549 impairs the tumorigenic capacity of TGES-1 cells. (B) The effect of SH-circ0000549-EX treatment on tumor weight. (C) The effect of SH-circ0000549-EX treatment on tumor volume. (D) The level of circ0000549 in tumor tissues after SH-circ0000549-EX treatment by RT-qPCR. (E) The levels of stemness, EMT and proliferation proteins in tumor tissues after SH-circ0000549-EX treatment. (F) The mRNA expression of stemness, EMT and proliferation makers mRNA in tumor tissues after SH-circ0000549-EX treatment. (G) Immunohistochemical analysis of tumor tissues treated with SH-circ0000549-EX revealed alterations in the levels of EMT and stemness markers, magnification, ×400. (H) The effect of OE-circ0000549-EX alone or in combination with miR-15b-5p mimic on the tumorigenicity of TGES-1 cells. (I) The effect of OE-circ0000549-EX alone or in combination with miR-15b-5p mimic on tumor weight and volume. (J) The levels of stemness, EMT and proliferation-related markers in tumor tissues following treatment with OE-circ0000549-EX alone or in combination with miR-15b-5p mimic. (K) The expression of stemness, EMT and proliferation mRNA in tumor tissues after treated with OE-circ0000549-EX alone or in combination with miR-15b-5p mimic. (L) The levels <t>of</t> <t>vimentin</t> and <t>Nanog</t> in tumor tissues after SH-circ0000549-EX treatment by Immunohistochemistry, magnification, ×200. * P<0.05, ** P<0.01, *** P<0.001, # P<0.05, ## P<0.01. EMT, epithelial-mesenchymal transition; RT-qPCR, reverse transcription-quantitative PCR; NC, negative control; SH-circ549-EX, exosomes from TGES-1 cells treated with circ0000549 lentivirus knockdown; SH-NC-EX; exosomes from TGES-1 cells treated with circ0000549 lentivirus knockdown negative control; OE-circ549-EX, exosomes from TGES-1 cells treated with circ0000549 lentivirus overexpression.
    Nanog, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 630 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+nanog/Nanog+XP+Rabbit+mAb/pmc12974335-190-22-23
    Average 96 stars, based on 630 article reviews
    nanog - by Bioz Stars, 2026-09
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    Images

    1) Product Images from "Exosomal circ0000549 promotes MNNG-induced gastric cancer through miR-15b-5p/KIF1B"

    Article Title: Exosomal circ0000549 promotes MNNG-induced gastric cancer through miR-15b-5p/KIF1B

    Journal: International Journal of Molecular Medicine

    doi: 10.3892/ijmm.2026.5785

    Exosomal circ0000549 tumorigenicity, EMT, proliferation and stemness. (A) The downregulation of circ0000549 impairs the tumorigenic capacity of TGES-1 cells. (B) The effect of SH-circ0000549-EX treatment on tumor weight. (C) The effect of SH-circ0000549-EX treatment on tumor volume. (D) The level of circ0000549 in tumor tissues after SH-circ0000549-EX treatment by RT-qPCR. (E) The levels of stemness, EMT and proliferation proteins in tumor tissues after SH-circ0000549-EX treatment. (F) The mRNA expression of stemness, EMT and proliferation makers mRNA in tumor tissues after SH-circ0000549-EX treatment. (G) Immunohistochemical analysis of tumor tissues treated with SH-circ0000549-EX revealed alterations in the levels of EMT and stemness markers, magnification, ×400. (H) The effect of OE-circ0000549-EX alone or in combination with miR-15b-5p mimic on the tumorigenicity of TGES-1 cells. (I) The effect of OE-circ0000549-EX alone or in combination with miR-15b-5p mimic on tumor weight and volume. (J) The levels of stemness, EMT and proliferation-related markers in tumor tissues following treatment with OE-circ0000549-EX alone or in combination with miR-15b-5p mimic. (K) The expression of stemness, EMT and proliferation mRNA in tumor tissues after treated with OE-circ0000549-EX alone or in combination with miR-15b-5p mimic. (L) The levels of vimentin and Nanog in tumor tissues after SH-circ0000549-EX treatment by Immunohistochemistry, magnification, ×200. * P<0.05, ** P<0.01, *** P<0.001, # P<0.05, ## P<0.01. EMT, epithelial-mesenchymal transition; RT-qPCR, reverse transcription-quantitative PCR; NC, negative control; SH-circ549-EX, exosomes from TGES-1 cells treated with circ0000549 lentivirus knockdown; SH-NC-EX; exosomes from TGES-1 cells treated with circ0000549 lentivirus knockdown negative control; OE-circ549-EX, exosomes from TGES-1 cells treated with circ0000549 lentivirus overexpression.
    Figure Legend Snippet: Exosomal circ0000549 tumorigenicity, EMT, proliferation and stemness. (A) The downregulation of circ0000549 impairs the tumorigenic capacity of TGES-1 cells. (B) The effect of SH-circ0000549-EX treatment on tumor weight. (C) The effect of SH-circ0000549-EX treatment on tumor volume. (D) The level of circ0000549 in tumor tissues after SH-circ0000549-EX treatment by RT-qPCR. (E) The levels of stemness, EMT and proliferation proteins in tumor tissues after SH-circ0000549-EX treatment. (F) The mRNA expression of stemness, EMT and proliferation makers mRNA in tumor tissues after SH-circ0000549-EX treatment. (G) Immunohistochemical analysis of tumor tissues treated with SH-circ0000549-EX revealed alterations in the levels of EMT and stemness markers, magnification, ×400. (H) The effect of OE-circ0000549-EX alone or in combination with miR-15b-5p mimic on the tumorigenicity of TGES-1 cells. (I) The effect of OE-circ0000549-EX alone or in combination with miR-15b-5p mimic on tumor weight and volume. (J) The levels of stemness, EMT and proliferation-related markers in tumor tissues following treatment with OE-circ0000549-EX alone or in combination with miR-15b-5p mimic. (K) The expression of stemness, EMT and proliferation mRNA in tumor tissues after treated with OE-circ0000549-EX alone or in combination with miR-15b-5p mimic. (L) The levels of vimentin and Nanog in tumor tissues after SH-circ0000549-EX treatment by Immunohistochemistry, magnification, ×200. * P<0.05, ** P<0.01, *** P<0.001, # P<0.05, ## P<0.01. EMT, epithelial-mesenchymal transition; RT-qPCR, reverse transcription-quantitative PCR; NC, negative control; SH-circ549-EX, exosomes from TGES-1 cells treated with circ0000549 lentivirus knockdown; SH-NC-EX; exosomes from TGES-1 cells treated with circ0000549 lentivirus knockdown negative control; OE-circ549-EX, exosomes from TGES-1 cells treated with circ0000549 lentivirus overexpression.

    Techniques Used: Quantitative RT-PCR, Expressing, Immunohistochemical staining, Immunohistochemistry, Reverse Transcription, Real-time Polymerase Chain Reaction, Negative Control, Knockdown, Over Expression

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    Cell Signaling Technology Inc anti nanog
    Exosomal circ0000549 tumorigenicity, EMT, proliferation and stemness. (A) The downregulation of circ0000549 impairs the tumorigenic capacity of TGES-1 cells. (B) The effect of SH-circ0000549-EX treatment on tumor weight. (C) The effect of SH-circ0000549-EX treatment on tumor volume. (D) The level of circ0000549 in tumor tissues after SH-circ0000549-EX treatment by RT-qPCR. (E) The levels of stemness, EMT and proliferation proteins in tumor tissues after SH-circ0000549-EX treatment. (F) The mRNA expression of stemness, EMT and proliferation makers mRNA in tumor tissues after SH-circ0000549-EX treatment. (G) Immunohistochemical analysis of tumor tissues treated with SH-circ0000549-EX revealed alterations in the levels of EMT and stemness markers, magnification, ×400. (H) The effect of OE-circ0000549-EX alone or in combination with miR-15b-5p mimic on the tumorigenicity of TGES-1 cells. (I) The effect of OE-circ0000549-EX alone or in combination with miR-15b-5p mimic on tumor weight and volume. (J) The levels of stemness, EMT and proliferation-related markers in tumor tissues following treatment with OE-circ0000549-EX alone or in combination with miR-15b-5p mimic. (K) The expression of stemness, EMT and proliferation mRNA in tumor tissues after treated with OE-circ0000549-EX alone or in combination with miR-15b-5p mimic. (L) The levels <t>of</t> <t>vimentin</t> and <t>Nanog</t> in tumor tissues after SH-circ0000549-EX treatment by Immunohistochemistry, magnification, ×200. * P<0.05, ** P<0.01, *** P<0.001, # P<0.05, ## P<0.01. EMT, epithelial-mesenchymal transition; RT-qPCR, reverse transcription-quantitative PCR; NC, negative control; SH-circ549-EX, exosomes from TGES-1 cells treated with circ0000549 lentivirus knockdown; SH-NC-EX; exosomes from TGES-1 cells treated with circ0000549 lentivirus knockdown negative control; OE-circ549-EX, exosomes from TGES-1 cells treated with circ0000549 lentivirus overexpression.
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    Exosomal circ0000549 tumorigenicity, EMT, proliferation and stemness. (A) The downregulation of circ0000549 impairs the tumorigenic capacity of TGES-1 cells. (B) The effect of SH-circ0000549-EX treatment on tumor weight. (C) The effect of SH-circ0000549-EX treatment on tumor volume. (D) The level of circ0000549 in tumor tissues after SH-circ0000549-EX treatment by RT-qPCR. (E) The levels of stemness, EMT and proliferation proteins in tumor tissues after SH-circ0000549-EX treatment. (F) The mRNA expression of stemness, EMT and proliferation makers mRNA in tumor tissues after SH-circ0000549-EX treatment. (G) Immunohistochemical analysis of tumor tissues treated with SH-circ0000549-EX revealed alterations in the levels of EMT and stemness markers, magnification, ×400. (H) The effect of OE-circ0000549-EX alone or in combination with miR-15b-5p mimic on the tumorigenicity of TGES-1 cells. (I) The effect of OE-circ0000549-EX alone or in combination with miR-15b-5p mimic on tumor weight and volume. (J) The levels of stemness, EMT and proliferation-related markers in tumor tissues following treatment with OE-circ0000549-EX alone or in combination with miR-15b-5p mimic. (K) The expression of stemness, EMT and proliferation mRNA in tumor tissues after treated with OE-circ0000549-EX alone or in combination with miR-15b-5p mimic. (L) The levels of vimentin and Nanog in tumor tissues after SH-circ0000549-EX treatment by Immunohistochemistry, magnification, ×200. * P<0.05, ** P<0.01, *** P<0.001, # P<0.05, ## P<0.01. EMT, epithelial-mesenchymal transition; RT-qPCR, reverse transcription-quantitative PCR; NC, negative control; SH-circ549-EX, exosomes from TGES-1 cells treated with circ0000549 lentivirus knockdown; SH-NC-EX; exosomes from TGES-1 cells treated with circ0000549 lentivirus knockdown negative control; OE-circ549-EX, exosomes from TGES-1 cells treated with circ0000549 lentivirus overexpression.

    Journal: International Journal of Molecular Medicine

    Article Title: Exosomal circ0000549 promotes MNNG-induced gastric cancer through miR-15b-5p/KIF1B

    doi: 10.3892/ijmm.2026.5785

    Figure Lengend Snippet: Exosomal circ0000549 tumorigenicity, EMT, proliferation and stemness. (A) The downregulation of circ0000549 impairs the tumorigenic capacity of TGES-1 cells. (B) The effect of SH-circ0000549-EX treatment on tumor weight. (C) The effect of SH-circ0000549-EX treatment on tumor volume. (D) The level of circ0000549 in tumor tissues after SH-circ0000549-EX treatment by RT-qPCR. (E) The levels of stemness, EMT and proliferation proteins in tumor tissues after SH-circ0000549-EX treatment. (F) The mRNA expression of stemness, EMT and proliferation makers mRNA in tumor tissues after SH-circ0000549-EX treatment. (G) Immunohistochemical analysis of tumor tissues treated with SH-circ0000549-EX revealed alterations in the levels of EMT and stemness markers, magnification, ×400. (H) The effect of OE-circ0000549-EX alone or in combination with miR-15b-5p mimic on the tumorigenicity of TGES-1 cells. (I) The effect of OE-circ0000549-EX alone or in combination with miR-15b-5p mimic on tumor weight and volume. (J) The levels of stemness, EMT and proliferation-related markers in tumor tissues following treatment with OE-circ0000549-EX alone or in combination with miR-15b-5p mimic. (K) The expression of stemness, EMT and proliferation mRNA in tumor tissues after treated with OE-circ0000549-EX alone or in combination with miR-15b-5p mimic. (L) The levels of vimentin and Nanog in tumor tissues after SH-circ0000549-EX treatment by Immunohistochemistry, magnification, ×200. * P<0.05, ** P<0.01, *** P<0.001, # P<0.05, ## P<0.01. EMT, epithelial-mesenchymal transition; RT-qPCR, reverse transcription-quantitative PCR; NC, negative control; SH-circ549-EX, exosomes from TGES-1 cells treated with circ0000549 lentivirus knockdown; SH-NC-EX; exosomes from TGES-1 cells treated with circ0000549 lentivirus knockdown negative control; OE-circ549-EX, exosomes from TGES-1 cells treated with circ0000549 lentivirus overexpression.

    Article Snippet: Subsequently, the sections were incubated overnight at 4°C with primary antibodies against Vimentin (Cell Signaling Technology, Inc; cat. no. 5741S; dilution:1:500) and Nanog (Cell Signaling Technology, Inc; cat. no. 4903S; dilution:1:500).

    Techniques: Quantitative RT-PCR, Expressing, Immunohistochemical staining, Immunohistochemistry, Reverse Transcription, Real-time Polymerase Chain Reaction, Negative Control, Knockdown, Over Expression